Active microglia/macrophage, surrounding and within the peri-infarct areas, expressed YM1, a marker of M2 microglia/macrophage activation, at four weeks. Increased MMP-2 and −3 were detected at four weeks. Dynamic contrast-enhanced MRI indicated that minocycline reduced BBB permeability accompanied with higher levels of TJPs measured with Western blot. Cerebral blood flow measured with arterial spin labeling (ASL) showed that minocycline improved perfusion. Minocycline significantly reduced the infarct size and prevented tissue loss in the ischemic hemispheres compared to vehicle-treated rats from two to four weeks as measured with MRI. ![]() ![]() They were studied at multiple time points up to four weeks with magnetic resonance imaging (MRI), immunohistochemistry and biochemistry. At the onset of reperfusion they received a single dose of minocycline (3 mg/kg intravenously) or a vehicle. MethodsĪdult spontaneously hypertensive rats had a 90 minute transient middle cerebral artery occlusion. In the present study, we longitudinally investigated neurological outcome, neurovascular remodeling and microglia/macrophage alternative activation after spontaneous and minocycline-induced stroke recovery. We have shown that spontaneous angiogenesis vessels in ischemic brain regions have high blood–brain barrier (BBB) permeability due to lack of major tight junction proteins (TJPs) in endothelial cells at three weeks. Prior studies of minocycline investigated short-term neuroprotective effects during subacute stage of stroke however, the late effects of minocycline against early reperfusion injury on neurovascular remodeling are less well studied. : loci/common/DebugToolsĪt (LociImporter.java:69)Īt HandleExtraFileTypes.openImage(HandleExtraFileTypes.java:499)Īt n(HandleExtraFileTypes.java:72)Īt ij.io.Opener.openWithHandleExtraFileTypes(Opener.java:516)Īt ij.io.Opener.openImage(Opener.java:370)Īt ij.io.Opener.openImage(Opener.java:242)Īt ij.io.Opener.openAndAddToRecent(Opener.java:291)Īt 1Helper.openAndAddToRecent(IJ1Helper.java:465)Īt 1Helper$LegacyEventDelegator.onEvent(IJ1Helper.java:591)Īt 0(Native Method)Īt (NativeMethodAccessorImpl.java:62)Īt (DelegatingMethodAccessorImpl.java:43)Īt .invoke(Method.java:498)Īt $ProxySubscriber.onEvent(DefaultEventService.java:302)Īt $ProxySubscriber.onEvent(DefaultEventService.java:276)Īt .ThreadSafeEventService.publish(ThreadSafeEventService.java:971)Īt .access$101(DefaultEventBus.java:56)Īt $1.run(DefaultEventBus.java:190)Īt .invoke(DefaultThreadService.java:107)Īt .publishNow(DefaultEventBus.java:184)Īt .publishNow(DefaultEventBus.java:75)Īt .publish(DefaultEventService.java:104)Īt .(OSXAppEventDispatcher.java:205)Īt ._AppEventHandler$_OpenFileDispatcher.performUsing(_AppEventHandler.java:361)Īt ._AppEventHandler$_OpenFileDispatcher.performUsing(_AppEventHandler.java:352)Īt ._AppEventHandler$_AppEventDispatcher$1.run(_AppEventHandler.java:516)Īt .dispatch(InvocationEvent.java:311)Īt (EventQueue.java:756)Īt $500(EventQueue.java:97)Īt $3.run(EventQueue.java:709)Īt $3.run(EventQueue.java:703)Īt (Native Method)Īt $JavaSecurityAccessImpl.doIntersectionPrivilege(ProtectionDomain.java:80)Īt (EventQueue.java:726)Īt (EventDispatchThread.java:201)Īt (EventDispatchThread.java:116)Īt (EventDispatchThread.java:105)Īt (EventDispatchThread.java:101)Īt (EventDispatchThread.java:93)Īt (EventDispatchThread.Minocycline reduces reperfusion injury by inhibiting matrix metalloproteinases (MMPs) and microglia activity after cerebral ischemia. ![]() ![]() After updating Fiji, I can not open my old file of “Lif”.
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